马铃薯卷叶病毒,potato leafroll virus
1)potato leafroll virus马铃薯卷叶病毒
1.Using RT-PCR-RFLP to detect and analysispotato leafroll virus;马铃薯卷叶病毒RT-PCR-RFLP的检测分析
2.The cDNA Cloning and Nucleotide Sequence Analysis of Potato Leafroll Virus Intergenic Region;马铃薯卷叶病毒基因间隔区的克隆及序列分析
3.One-step reverse-transcription polymerase chain reaction(RT-PCR) protocol for detection of potato virus X,potato virus Y andpotato leafroll virus in infected potatoes and tobaccoes was developed.运用一步法RT-PCR对马铃薯和烟草中的马铃薯X病毒、马铃薯Y病毒和马铃薯卷叶病毒进行了检测,实验表明:对酚-SDS方法提取的病毒核酸进行扩增,一步法RT-PCR检测到的扩增产物的灵敏度较两步法RT-PCR的高100倍左右。
英文短句/例句
1.Construction of Double-stranded RNA Interference Vector Against Potato Leafroll Virus;马铃薯卷叶病毒双链RNA干涉载体构建
2.Study on Detection of PVY and PLRV Using Taqman Real-Time Quantitative PCR马铃薯Y病毒和马铃薯卷叶病毒的实时荧光同步检测技术的研究
3.Research on the detection of PVA and PLRV by Duplex RT-PCR双重RT-PCR同步检测马铃薯A病毒和马铃薯卷叶病毒
4.The Molecular Identification and Detection Methods of Potatoleafroll Viruse;马铃薯卷叶病毒的分子鉴定与检测技术
5.Cloning, Prokaryotic Expression of PLRV CP Gene and Preparation of Antiserum;马铃薯卷叶病毒CP基因的克隆、原核表达及抗血清的制备
6.Study on Genetic Diversity of Germplasm Resources in Sugarbeet and the Detection of Ribozyme Gene in Transgene Potato;甜菜种质资源的遗传多样性研究与马铃薯卷叶病毒核酶的检测
7.Construction of Expression vector Vector of Deletion Mutation CP Gene of Potato Leafroll Virus for Saccharomyces cerevisiae马铃薯卷叶病毒缺失突变CP基因酿酒酵母表达载体的构建
8.Studies on Principles of the Inhibition to Photosynthesis after Potato Virus Y Infection;马铃薯Y病毒侵染对马铃薯叶片光合抑制机理的研究
9.mosaic-type degeneration of potato马铃薯花叶型退化病
10.Identification of a New Menber of Genus Potyvirus--Thunberg Fritillary Mosaic Virus;一个侵染浙贝母的马铃薯Y病毒属新成员——浙贝母花叶病毒的鉴定
11.Molecular Variability and Synergism of Potato Virus X and Tobacco Vein Banding Mosaic Virus;马铃薯X病毒和烟草脉带花叶病毒的分子变异及协生作用
12.Molecular Characterization of Turnip Mosaic Virus, Potato Virus Y and Tobacco Vein-banding Mosaic Virus in China;三种马铃薯Y病毒属病毒的分子特性
13.In Vitro Tissue Culture and Virus-Free Detection of Potato;马铃薯组织培养脱毒和病毒检测研究
14.Epidemic Survey on Potato Virus and Detection and Identification of Potato Virus S in Zhangjiakou City of Hebei Province马铃薯病毒疫情调查及马铃薯S病毒的检测鉴定
15.Advance of Virus Detection of Virus-Free Seed(Seedling) Potatoes马铃薯脱毒种薯(苗)病毒检测技术研究进展
16.Simultaneous Detection of Multiple Potato Viruses with Multiplex RT-PCR;马铃薯病毒多重RT-PCR检测技术研究
17.RNA-Mediated Virus Resistance in Transgenic Potato;RNA介导的转基因马铃薯抗病毒研究
18.A Study on the one-step RT-PCR for detection of potato Viruses;马铃薯病毒一步法RT-PCR诊断研究
相关短句/例句
potato leafroll virus (PLRV)马铃薯卷叶病毒
1.Total RNA of potato leafroll virus (PLRV) was extracted from virus-infected potato leaves collected from fields in Zhangjiakou of Hebei.从典型的马铃薯卷叶病毒病株中直接提取出植物总RNA ,根据PLRV外壳蛋白基因序列 ,设计合成了一对寡核苷酸引物 ,经RT -PCR法扩增出全长的cDNA片段 ,将其克隆到质粒pGEM - 3Zf (+)上 ,并进行了序列分析。
2.The intergenic sequence (IS) cDNA of Potato Leafroll Virus (PLRV) Chinese isolate was constructed into transformation binary vector pROK2 in positive or negative direction, then introduced into the commercial potato cultivar Desiree via Agrobacterium tumefaciens mediated gene transfer.将本室合成、克隆的马铃薯卷叶病毒（ＰｏｔａｔｏＬｅａｆｒｏｌＶｉｒｕｓ，ＰＬＲＶ）中国分离株的基因间隔区（ｉｎｔｅｒｇｅｎｉｃｓｅｑｕｅｎｃｅ，ＩＳ）双链ｃＤＮＡ以正、反向两种方式分别构建于转化载体ｐＲＯＫ２中，通过致瘤农杆菌介导，以马铃薯叶圆片为转化材料，转化马铃薯栽培品种Ｄｅｓｉｒｅ，获得了转基因植株。
3)PLRV马铃薯卷叶病毒
1.A pair of DNA primers were designed and synthesized based on the nucleotide sequence of potato leafroll virus(PLRV).根据马铃薯卷叶病毒(PLRV)外壳蛋白基因的保守序列设计了一对寡聚核苷酸引物,从田间自然感染PLRV的马铃薯病株中提取病毒总RNA,用反转录-聚合酶链式反应扩增出符合设计大小240bp的特异性产物,而对照没有任何扩增产物。
4)The genus Polerovirus马铃薯卷叶病毒属
5)Potato leafroll virus马铃薯卷叶病毒(PLRV)
6)potato virus A马铃薯A病毒
1.The clone and sequence analysis on the CP gene ofpotato virus A;马铃薯A病毒CP基因的克隆与序列分析
2.Determination and phylogenetic tree analysis of 3 -part genomic sequence of apotato virus A isolates occurred in Hangzhou;杭州郊区马铃薯A病毒分离物的基因组3’-末端序列测定及系统进化分析
3.According to the nucleotide and amino acid sequences of coat protein( CP), the Potato virus A (PVA)was identified in Fujian.依据马铃薯A病毒(PotatovirusA,PVA)外壳蛋白(coatprotein,CP)基因核苷酸序列及氨基酸序列对福建省PVA进行了鉴定。
延伸阅读
马铃薯泥土司卷全麦土司 4片马铃薯泥 300公克奶油起司 150公克砂糖 80公克柠檬汁 20cc彩色餐巾纸 1张魔带 1段制作：1.奶油起司用打蛋器拌软，加入马铃薯泥拌匀，再加入砂糖及柠檬汁充分混拌即可。2.全麦土司铺上作法1中的马铃薯泥，以捲寿司的方式捲起，用餐巾纸包住，繫上魔带即可。捲土司时可先铺上一张玻璃纸，再将土司捲起，一来方便携带，二来可以防止土司失水变乾。本食谱可做4份。
