疏水作用色谱,hydrophobic interaction chromatography
1)hydrophobic interaction chromatography疏水作用色谱
1.Almost all the lipid in fresh hemolysate of bovine red blood was removed withhydrophobic interaction chromatography (HIC) in the presence of 2%PEG4000, 5%PEG4000, 2%PEG10000 or 5%PEG10000.经过疏水作用色谱后 ,血红蛋白的P5 0是 3386 4Pa,Hill系数是 2 。
2.AIM: To investigate the refolding of the reduced/denatured ribonuclease (RNase) by high performancehydrophobic interaction chromatography (HPHIC) and high performance ion exchange chromatography (HPIEC) and to study the factors influencing its refolding.目的:研究还原变性的核糖核酸酶(RNase)在高效疏水作用色谱(HPHIC)及高效离子交换色谱(HPIEC)上的复性及影响因素。
英文短句/例句
1.Thermodynamics of retention for α-amylase in hydrophobic interaction chromatographyα-淀粉酶在疏水作用色谱中保留的热力学研究(英文)
2.Effect of Hydrophobic Interaction on Retention of Ion-exchange Chromatography;疏水作用对离子交换色谱保留影响的研究
3.Abstract： Results from a silica-based HIC stationary phase with multifunctional attachment of amide groups are presented.本文介绍了一种以硅胶为基质，修饰了多功能氨基官能团的疏水相互作用色谱固定相。
4.Studies of Adsorption Equilibrium and Kinetics of Hydrophobic Charge Induction Chromatography;疏水电荷诱导色谱的吸附平衡及动力学研究
5.The Ligand Screening and Fabrication of Adsorbent for Hydrophobic Charge Induction Chromatography;新型疏水电荷诱导色谱配基的筛选与介质制备
6.Thermodynamics of the Retention for Proteins in Hydrophobic Interaction Chromatography;在疏水色谱中蛋白质保留的热力学研究
7.Stationary phases for hydrophilic interaction liquid chromatography and their applications in separation of traditional Chinese medicines亲水作用色谱固定相及其在中药分离中的应用
8.hydrophobic collapse疏水折拢[由疏水作用而引起肽链的折叠]
9.Refoding and Purification of Recombinant Human Granulocyte-Macrophage Colony Stimulating Factor with Hydrophobic Interaction Chromatography用疏水色谱法复性并同时纯化重组人粒细胞-巨噬细胞集落刺激因子
10.Determination of streptomycin and dihydrostreptomycin in honey by hydrophilic interaction chromatography combined with tandem-mass spectrometry亲水作用色谱-串联质谱法测定蜂蜜中的链霉素和双氢链霉素(英文)
11.Determination of Cyromazine and Melamine Residues in Vegetables by Triple Quadurupole Liquid Chromatography-Tandem Mass Spectrometry with Hydrophilic Interaction Chromatography亲水作用色谱-串联质谱测定蔬菜中灭蝇胺及其代谢物三聚氰胺
12.Sorption and Transport of Hydrophobic Organic Compounds in Soil-Water System Studied by Liquid Chromatographic Method;疏水有机化合物在土壤—水体系中吸着、迁移的液相色谱法研究
13.Study of Adsorption of Water and Alcohols on Hydrophobic Porous Silica by Pulse Chromatography脉冲色谱法研究醇和水在疏水多孔硅胶上的吸附行为
14.The thermodynamics study on the retention of proteins and aromatic alcohol homologues in hydrophobic interaction chromatography;蛋白质和芳香醇同系物在疏水色谱中保留的热力学研究
15.Study on Microemulsion Electrokinetic Chromatography for Hydrophobicity of Pharmaceuticals;药物疏水性微乳液毛细管电动色谱评价方法研究
16.Hydrophobic Charge Induction Chromatography: Ligand Screening, Coupling and Its Thermodynamic Analysis;新型疏水电荷诱导色谱配基的筛选、偶联及热力学研究
17.Study on Low-Temperature Thermal Desorption of Hydrophobic Organic Pollutants from Soil Using Gas Chromatographic Method;土壤中疏水性有机污染物低温热脱附过程的气相色谱法研究
18.Solid Phase Extraction-Hydrophilic Interaction Chromatographic Determination of Tetracycline Antibiotics in Wastewater固相萃取-亲水作用色谱法测定废水中四环素类抗生素
相关短句/例句
high performance hydrophobic interaction chromatography(HPHIC)高效疏水作用色谱法
3)Hydrophobic interaction chromatography疏水相互作用色谱
1.A chromatographic procedure including anion exchange chromatography and hydrophobic interaction chromatography has been developed to separate and purify 90 kD elicitin in culture of P.利用阴离子交换色谱和疏水相互作用色谱从烟草疫霉菌培养液中分离了一种新的９０ＫＤ激发子蛋白，讨论了阴离子交换色谱流动相的最佳ｐＨ值，建立了疏水相互作用色谱，硫酸铵与三羟甲基氨基甲烷（Ｔｒｉｓ）缓冲液/水洗脱模式，简化了纯化步骤和活性损失的危险。
2.The thermodynamic behaviors for some proteins and small molecules in hydrophobic interaction chromatography are studied in the temperature range of 21-80℃.研究21～80℃温度范围内一些蛋白质和小分子在疏水相互作用色谱中的热行为。
3.Based on the fact that the resolution of intact protein separation is almost independent of column length,the effect on the resolution for intact protein separation causing from dynamic factors in hydrophobic interaction chromatography(HIC) was investigated.依据液相色谱分离整体蛋白的效果与色谱柱柱长基本无关的事实,研究了动力学因素对疏水相互作用色谱(HIC)分离整体蛋白的影响。
4)hydrophobic interaction chromatography疏水相互作用色谱法
1.The development ofhydrophobic interaction chromatography (HIC) is mainly concerned in this article.综合评述了疏水相互作用色谱法 ( hydrophobic interaction chromatography,HIC)的理论及其在生物大分子分离纯化方面的研究进
5)hydrophobic interaction chromatographic packing materials疏水作用色谱填料
6)high performance hydrophobic interaction chromatography高效疏水作用色谱
1.A protocol comprising PEG8000 precipitation,high performance hydrophobic interaction chromatography (HPHIC) and high performance ion exchange chromatography (HPIEC) for separation and purification of cytochrome P450 in the cotton bollworm, Helicoverpa armigera, was introduced.作者建立了适用于棉铃虫中肠微粒体P450的纯化方法,包括聚乙二醇8000(PEG8000)沉淀、高效疏水作用色谱(HPHIC)和高效离子交换色谱(HPIEC)等连续分离步骤。
延伸阅读
疏水作用分子式：CAS号：性质：又称为憎水作用，冰山结构（iceberg structure）。在某些大离子的水溶液中，如四级烷基铵盐及肥皂的水溶液中，离子的电荷中心上的功能团与水分子相互作用的倾向，小于水分子之间相互作用时，出现疏水作用。此作用可分为两种情况。在无限稀释水溶液中，溶质与水之间的疏水作用为本征疏水相互作用，亦称为负水化。在一定浓度的水溶液中，两个或两个以上疏水溶质分子之间相互作用，发生溶质粒子的缔合，从而影响溶液的热力学性质，也为疏水作用。此作用对水溶液表面吸附、胶体电解质胶团形成以上溶液中蛋白质的构象都起着关键作用。
