实时定量聚合酶链式反应,real-time quantitative polymerase chain reaction(RQ-PCR)
1)real-time quantitative polymerase chain reaction(RQ-PCR)实时定量聚合酶链式反应
1.The mRNA expression levels of important insulin signal molecules in rat muscle of alloxan-induced diabetes were studied by real-time quantitative polymerase chain reaction(RQ-PCR).运用实时定量聚合酶链式反应(RQ-PCR)研究了正常以及患有糖尿病大鼠的肌肉组织中胰岛素信号转导通路中重要的信号传导分子基因表达,结果表明:四氧嘧啶诱导的糖尿病大鼠肌肉组织的胰岛素受体、胰岛素受体底物1和葡萄糖转运体4的mRNA表达下调,胰岛素磷脂酰肌醇3激酶的mRNA表达上调;糖尿病大鼠骨骼肌葡萄糖转运体4的mRNA含量下降可能会引起糖摄取能力的下降,从而导致四氧嘧啶诱导大鼠肌肉组织胰岛素抵抗。
2)Real-Time Quantitative polymerase chain reaction实时定量聚合酶链反应
1.The Establishment of Real-time Quantitative Polymerase Chain Reaction Method for Detecting Cardiovascular Bioactive Polypeptide Apelin;实时定量聚合酶链反应检测心血管活性多肽apelin方法的建立
英文短句/例句
1.Detection of mtDNA 4 977 bp deletion in peripheral whole-blood of healthy adult健康人周围全血线粒体DNA4977bp缺失的实时定量聚合酶链反应检测
2.Detection of Yersinia enterocolitica in diarrhea stool by real-time PCR实时定量聚合酶链反应检测腹泻粪便中小肠结肠炎耶尔森菌的研究与评价
3.Research of Real-time Quantitative Reverse-transcriptase Polymerase Chain Reaction (RQ-PCR) to Detect bcr/abl Fusion Gene in Chronic Myeloid Leukemia;实时定量逆转录聚合酶链反应检测慢粒bcr/abl融合基因的研究
4.Detection of mutans streptococci in children saliva by real-time fluorescence quantitative polymerase chain reaction methods;实时荧光定量聚合酶链反应检测儿童口腔致龋菌的实验研究
5.The Preparation of Satb2 Gene Standard Plasmids for Real-time Quantitative PCR and Its Application;Satb2基因实时荧光定量聚合酶链反应标准品的制备及应用研究
6.Monitoring of Minimal Residual Disease (MRD) by Real-time Quantitative Reverse Transcription PCR in Acute Promyelocytic Leukemia;实时荧光定量聚合酶链反应对人急性早幼粒细胞白血病的MRD监测
7.EXPERIMENTAL STUDY ON CALORIMETRY OF POLYMERASE CHAIN REACTION聚合酶链式反应(PCR)量热学的实验研究
8.The assay method used included the Roche Amplicor assay (version 2.0) and real-time PCR.检测方法采用实时荧光定量聚合酶链反应方法和罗氏公司的pcr内标定量方法。
9.Effects of cyclic tension force on the expression of BGLAP in human periodontal ligament cells实时定量反转录-聚合酶链反应检测机械力对人牙周膜细胞骨钙素表达的影响
10.Apply of Real-time Fluorescence Quantitative Polymerase Chain Reaction for the Diagnosis of down Syndrome from a Single Cell;单细胞水平实时荧光定量聚和酶链反应在诊断唐氏综合征中的应用探讨
11.Monitoring of P16 Abnormal Methylation by Real-time Fluorescent Quantitative PCR in NSCLC;实时荧光定量聚合酶链反应检测人肺癌P16抑癌基因启动子异常甲基化
12.Study of TaqMan assay of real-time fluorescence quantitative PCR to detect Staphylococcus aureus in food实时荧光定量-聚合酶链反应检测食品中金黄色葡萄球菌方法的研究
13.Species identification of animal cells by polymerase chain reaction应用聚合酶链式反应鉴定实验细胞的来源种属
14.Detection of Campylobacter jejuni by real-time fluorescent PCR实时荧光聚合酶链反应检测空肠弯曲菌
15.The accidence exploration of estimation of curative effect in hepatitis B by fluorescence quantitative PCR荧光定量聚合酶链反应对乙型肝炎患者个体用药疗效的评定
16.Research on the Application in Diagnosis of Fluorescent Quantitation Polymerase Chain Reaction of Paratyphoid A荧光定量聚合酶链反应诊断甲型副伤寒的应用研究
17.Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型
18.Rapid detection of Shigella by real-time fluorescent PCR assay using TaqMan-MGB probes志贺菌TaqMan-MGB探针实时荧光聚合酶链反应快速检测方法的应用研究
相关短句/例句
Real-Time Quantitative polymerase chain reaction实时定量聚合酶链反应
1.The Establishment of Real-time Quantitative Polymerase Chain Reaction Method for Detecting Cardiovascular Bioactive Polypeptide Apelin;实时定量聚合酶链反应检测心血管活性多肽apelin方法的建立
3)real-time quantitative PCR实时定量聚合酶链反应
1.Quantitative study on C/EBPζ gene transcripts in patients with chronic myeloid leukemia usingreal-time quantitative PCR;方法建立实时定量聚合酶链反应对76例不同阶段CML患者和16名正常对照的骨髓单个核细胞中C/EBPζ转录本含量进行检测。
4)Real time-PCR实时定量-聚合酶链反应
5)real time quantity polymerase chain reaction (RTQ-PCR)实时定量-聚合酶链式反应(RTQ-PCR)
6)Real-time RTpolymerase chain reaction实时荧光定量逆转录聚合酶链式反应
延伸阅读
聚合酶链反应聚合酶链反应polymerase chain reaction,PCR是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA)片断大量增扩,达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体,因其假阳性率较高,故应谨慎使用。